Fig 1: MiR-489-3p inactivates Wnt/β-catenin signaling pathway. A Signaling pathway kits were used to assess the influence of miR-489-3p on Notch, Wnt, PI3K/Akt, Nanog, Hippo-YAP, Hedgehog and NF-κB signaling pathways. B TOP/FOP flash luciferase reporter assay evaluated the signal activity of Wnt pathway after the enhancement of miR-489-3p. C β-catenin expression in the cytoplasm and nucleus of H1299 and A549 cells was testified by western blot after the overexpression of miR-489-3p. D–E Western blot and qPCR were performed to testify the levels of c-myc and c-jun in H1299 and A549 cells after the overexpression of miR-489-3p. F–G Western blot and qPCR assessed the levels of β-catenin, CK1, APC, Axin and GSK3β in H1299 and A549 cells after the overexpression of miR-489-3p. **P < 0.01
Supplier Page from Abcam for Human Nanog ELISA Kit